The course will help students to understand the basic concepts of the standard polymerase chain reaction (PCR) technique and fundamentals of PCR. This includes learning about steps involved in the PCR / PCR cycling: denaturation, annealing, extension, components of the PCR reaction: DNA template, DNA polymerase, dNTPs, forward primer and reverse primer. Students will learn how to conduct a PCR experiment in the laboratory using different PCR amplification programs on thermal cyclers and also will get certain tips for successful gel electrophoresis and tips for troubleshooting gel electrophoresis results.

 Also attention will be paid to understanding how to design primers for different types of PCRs and the criteria required to design a DNA primer for PCR, and to the online programs usually used to design a DNA primer. 

 This course will be included in the curriculum.

• ПЛР силабус
• Syllabus PCR
• ПЛР і Дизайн Праймерів конспект лекцій
• Посилання тест, творче завдання і фідбек